Autolytic ‘Protoplast’ Release in Bacterium coli
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چکیده
منابع مشابه
Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening.
In an effect to broaden the application of the heat-inducible autolytic vector pUC18-cI857/p(R)-SRRz-rrnB previously developed, a new vector pUC18-cI857/p(R)(T41C)-SRRz-rrnB (pEAS-1b) was quantitatively characterized under various growth temperatures, heat induction temperatures and durations, and IPTG (isopropyl beta-d-thiogalactoside) induction times, after resolving its erratic lysis profile...
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The study of inheritance in bacteria has, for the most part, been confined to the investigation. of mutational changes in the course of clonal reproduction. With the exception of experiments on pneumococcus type transformations there have been few studies on the direct hereditary interaction of one bacterial type with another. The conception that bacteria have no sexual mode of reproduction is ...
متن کاملStudies on an autolytic substance produced by an aerobic sporeforming bacterium.
The capacity of many "surface-active" materials to cause lysis of bacterial cultures is a well documented fact. In addition to lytic activity by nonbiologic detergents, one finds that many biologically produced substances have the ability to destroy the structural integrity of living microorganisms. Lysozyme lyses susceptible bacteria by hydrolyzing certain mucopolysaccharides necessary to the ...
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The construction of high-yield laccase-producing fusant was achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions demonstrated that with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened by different characters between two parental strai...
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Mohan, Raam R. (Warner-Lambert Research Institute, Morris Plains, N.J.), Donald P. Kronish, Roland S. Pianotti, Ray L. Epstein, and Benjamin S. Schwartz. Autolytic mechanism for spheroplast formation in Bacillus cereus and Escherichia coli. J. Bacteriol. 90:1355-1364. 1965.-Spheroplasts of Bacillus cereus strain T and Escherichia coli B were prepared by incubating early log-phase cells in appro...
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ژورنال
عنوان ژورنال: Nature
سال: 1956
ISSN: 0028-0836,1476-4687
DOI: 10.1038/178993a0